Question 1

Restriction mapping is used to characterize cloned DNA.What does a restriction map tell the researcher about the cloned DNA?

Accepted Answer

A)  The size of the genome the cloned DNA was isolated from.
B)  The restricted conditions under which the organism can grow.
C)  The number of restriction sites for the specific restriction enzyme.
D)  The distances between restriction sites for the specific restriction enzyme.
E)  The number of sites and distance between them for the specific restriction enzyme.F) C) and D)
G) A) and E)

Question 2

Transgenic animals are useful in studying the effects of a gene product on the organism's phenotype.Briefly describe how you would study the effect of overexpression of a pancreatic enzyme using a transgenic animal.

Accepted Answer

The gene of interest can be inserted int...

Question 3

When choosing a restriction enzyme for use in recombinant DNA technologies,it is often preferred that the enzyme generate cohesive,or "sticky," ends.Why is this preferred?

Accepted Answer

A)  The sticky ends prevent the DNA from re- annealing to any DNA.
B)  The sticky ends make the DNA bind tighter to any cut DNA.
C)  The sticky ends stick to the purification medium making the fragments easier to purify.
D)  The sticky ends have hydrogen bonds that help re- anneal the cut DNA.
E)  The sticky ends do not have hydrogen bonds to help in re- annealing the cut DNA.F) D) and E)
G) B) and C)

Question 4

Due to single nucleotide polymorphisms SNPs) ,all humans have a slightly different nucleotide sequence for their genome.Restriction mapping is used in forensic sciences to match DNA evidence to a potential suspect of a crime.How does restriction mapping reduce the likelihood of misidentifying a suspect?

Accepted Answer

A)  SNPs change the genes that are expressed
B)  SNPs only remove restriction sites from the genome resulting in a unique "fingerprint" for each person
C)  SNPs add and remove restriction sites resulting in a unique "fingerprint" for each person
D)  SNPs only add new restriction sites to the genome resulting in a unique "fingerprint" for each personE) None of the above
F) A) and B)

Question 5

The generation of a knock- out organism generally requires all the following EXCEPT________ .

Accepted Answer

A)  a targeting vector
B)  knowledge of the sequence product
C)  knowledge of the target sequence
D)  a selectable marker
E)  embryonic stem cellsF) A) and B)
G) A) and D)

Question 6

Next- generation sequencing takes advantage of the concept of sequencing- by- synthesis.What is sequence- by- synthesis?

Accepted Answer

A)  The use of PCR to incorporate ddNTPs,followed by electrophoresis.
B)  The use of a multiwell system and microfluidics to detect incorporated nucleotides at each base.
C)  The use of firefly luciferase and pyrophosphate to report on nucleotide incorporation.
D)  The use of a single polymerase in a nanopore that reports on a single molecule of synthesized DNA.E) A) and D)
F) A) and C)

Question 7

When performing a sequencing reaction using Sanger sequencing,which ddNTPs must be included in the reaction?

Accepted Answer

A)  ddGTP
B)  ddCTP
C)  ddATP
D)  ddTTP
E)  all four ddNTPs must be presentF) B) and C)
G) A) and E)

Question 8

Briefly explain the three major steps in the polymerase chain reaction.

Accepted Answer

The first step is denaturation of the ta...

Question 9

Recognition sequences for restriction enzymes possess the unique quality of being the same when read 5' to 3' on either strand.What is this property called?

Accepted Answer

A)  recombination sequence
B)  palindromic sequence
C)  consensus sequence
D)  gene sequence
E)  origin sequenceF) A) and D)
G) A) and C)

Question 10

Which of the following best describes a cloning vector?

Accepted Answer

A)  The direction in which DNA is cloned.
B)  The fragment of DNA encoding a gene of interest.
C)  A DNA molecule that accepts DNA fragments and replicates the fragment in a host.
D)  A DNA molecule that accepts DNA fragments and degrades them in a host.E) C) and D)
F) None of the above

Question 11

Recombinant DNA technologies are methods used to do which of the following?

Accepted Answer

A)  induce homologous recombination inside a cell
B)  manipulate DNA for the study of specific sequences
C)  combine chromosomes to make them easier to study
D)  integrate DNA in computers to make them faster
E)  join together DNA molecules from the same sourceF) All of the above
G) C) and D)

Question 12

Protospacer adjacent motifs,PAMs,allow bacteria to do which of the following?

Accepted Answer

A)  recognize and integrate foreign DNA
B)  recognize and digest foreign DNA
C)  recognize proper spacing for bacterial transcription initiation
D)  recognize and digest their own DNAE) A) and D)
F) B) and C)

Question 13

Fluorescence in situ hybridization is another way to visualize the presence of a nucleotide sequence.Which of the following is an advantage of FISH over Northern and Southern blots?

Accepted Answer

A)  Probes are not needed in FISH.
B)  There are no advantages to FISH over Northern and Southern blots.
C)  Blotting is not used in FISH.
D)  Fluorophores are not used in Northern or Southern blots.E) B) and C)
F) A) and D)

Question 14

Transformation of cells using the technique of heat shock requires which of the following?

Accepted Answer

A)  the use of zinc ions
B)  brief electric shock
C)  brief exposure to elevated temperature
D)  the use of calcium and brief exposure to elevated temperature
E)  the use of calcium ionsF) All of the above
G) C) and E)

Question 15

Agarose gels separate DNA fragments based on what property?

Accepted Answer

A)  amount of adenine bases in the sequence
B)  charge of the DNA molecule
C)  amount of agarose in the DNA
D)  size of the fragmentE) All of the above
F) C) and D)

Question 16

Nucleic acid blotting is commonly used in molecular biology.Northern blotting is used to detect the presence of which type of nucleic acid?

Accepted Answer

A)  ribonucleic acids
B)  unnatural nucleic acids
C)  deoxyribonucleic acids
D)  triphosphate nucleic acids
E)  transfer ribonucleic acidsF) A) and E)
G) A) and B)

Question 17

ddATP,ddTTP,ddCTP,and ddGTP are labeled with red,green,yellow,and blue fluorescent dyes,respectively.A five- base read from a sequencing reaction produced the following color sequence,read by the computer: red,yellow,yellow,green,green.What is the sequence of the template DNA?

Accepted Answer

A)  TGGAA
B)  AAGGT
C)  ACCTT
D)  TTCCAE) All of the above
F) B) and C)

Question 18

When a 5- kb circular plasmid is digested with a restriction enzyme that has three recognition sites on the plasmid,how many bands can be visualized on an agarose gel?

Accepted Answer

A)  1
B)  2
C)  3
D)  4E) B) and C)
F) A) and D)

Question 19

Sanger sequencing is based on the order in which ddNTPs are added to a growing polynucleotide.Why are ddNTPs integral to the Sanger sequencing method?

Accepted Answer

A)  They have a 3' hydroxyl that allows for extension of the polynucleotide.
B)  They do not have a 2' hydroxyl,which does not allow the extension of the polynucleotide.
C)  They do not have a 3' hydroxyl,which does not allow the extension of the polynucleotide.
D)  They have a 2' hydroxyl that allows for extension of the polynucleotide.E) All of the above
F) C) and D)

Question 20

Genomic libraries are constructed from the fragments generated from a________ digest.

Accepted Answer

A)  incomplete
B)  random
C)  complete
D)  gene specificE) B) and C)
F) A) and D)

Exam 20: Recombinant Dna Technology

Sanger sequencing is based on the order in which ddNTPs are added to a growing polynucleotide.Why are ddNTPs integral to the Sanger sequencing method?

Correct Answer
verified

Which of the following best describes a cloning vector?

Correct Answer
verified

Correct Answer
verified

Transgenic animals are useful in studying the effects of a gene product on the organism's phenotype.Briefly describe how you would study the effect of overexpression of a pancreatic enzyme using a transgenic animal.

Correct Answer
verified
The gene of interest can be inserted int...
View Answer

Agarose gels separate DNA fragments based on what property?

Correct Answer
verified

Nucleic acid blotting is commonly used in molecular biology.Northern blotting is used to detect the presence of which type of nucleic acid?

Correct Answer
verified

When a 5- kb circular plasmid is digested with a restriction enzyme that has three recognition sites on the plasmid,how many bands can be visualized on an agarose gel?

Correct Answer
verified

When choosing a restriction enzyme for use in recombinant DNA technologies,it is often preferred that the enzyme generate cohesive,or "sticky," ends.Why is this preferred?

Correct Answer
verified

Genomic libraries are constructed from the fragments generated from a________ digest.

Correct Answer
verified

When performing a sequencing reaction using Sanger sequencing,which ddNTPs must be included in the reaction?

Correct Answer
verified

Next- generation sequencing takes advantage of the concept of sequencing- by- synthesis.What is sequence- by- synthesis?

Correct Answer
verified

The generation of a knock- out organism generally requires all the following EXCEPT________ .

Correct Answer
verified

Protospacer adjacent motifs,PAMs,allow bacteria to do which of the following?

Correct Answer
verified

Recombinant DNA technologies are methods used to do which of the following?

Correct Answer
verified

Recognition sequences for restriction enzymes possess the unique quality of being the same when read 5' to 3' on either strand.What is this property called?

Correct Answer
verified

Transformation of cells using the technique of heat shock requires which of the following?

Correct Answer
verified

Briefly explain the three major steps in the polymerase chain reaction.

Correct Answer
verified
The first step is denaturation of the ta...
View Answer

Restriction mapping is used to characterize cloned DNA.What does a restriction map tell the researcher about the cloned DNA?

Correct Answer
verified

Fluorescence in situ hybridization is another way to visualize the presence of a nucleotide sequence.Which of the following is an advantage of FISH over Northern and Southern blots?

Correct Answer
verified

ddATP,ddTTP,ddCTP,and ddGTP are labeled with red,green,yellow,and blue fluorescent dyes,respectively.A five- base read from a sequencing reaction produced the following color sequence,read by the computer: red,yellow,yellow,green,green.What is the sequence of the template DNA?

Correct Answer
verified

Exam 20: Recombinant Dna Technology

Sanger sequencing is based on the order in which ddNTPs are added to a growing polynucleotide.Why are ddNTPs integral to the Sanger sequencing method?

Correct AnswerverifiedShow Answer

Which of the following best describes a cloning vector?

Correct AnswerverifiedShow Answer

Correct AnswerverifiedShow Answer

Transgenic animals are useful in studying the effects of a gene product on the organism's phenotype.Briefly describe how you would study the effect of overexpression of a pancreatic enzyme using a transgenic animal.

Correct AnswerverifiedThe gene of interest can be inserted int...View AnswerShow Answer

Agarose gels separate DNA fragments based on what property?

Correct AnswerverifiedShow Answer

Nucleic acid blotting is commonly used in molecular biology.Northern blotting is used to detect the presence of which type of nucleic acid?

Correct AnswerverifiedShow Answer

When a 5- kb circular plasmid is digested with a restriction enzyme that has three recognition sites on the plasmid,how many bands can be visualized on an agarose gel?

Correct AnswerverifiedShow Answer

When choosing a restriction enzyme for use in recombinant DNA technologies,it is often preferred that the enzyme generate cohesive,or "sticky," ends.Why is this preferred?

Correct AnswerverifiedShow Answer

Genomic libraries are constructed from the fragments generated from a________ digest.

Correct AnswerverifiedShow Answer

When performing a sequencing reaction using Sanger sequencing,which ddNTPs must be included in the reaction?

Correct AnswerverifiedShow Answer

Next- generation sequencing takes advantage of the concept of sequencing- by- synthesis.What is sequence- by- synthesis?

Correct AnswerverifiedShow Answer

The generation of a knock- out organism generally requires all the following EXCEPT________ .

Correct AnswerverifiedShow Answer

Protospacer adjacent motifs,PAMs,allow bacteria to do which of the following?

Correct AnswerverifiedShow Answer

Recombinant DNA technologies are methods used to do which of the following?

Correct AnswerverifiedShow Answer

Recognition sequences for restriction enzymes possess the unique quality of being the same when read 5' to 3' on either strand.What is this property called?

Correct AnswerverifiedShow Answer

Transformation of cells using the technique of heat shock requires which of the following?

Correct AnswerverifiedShow Answer

Briefly explain the three major steps in the polymerase chain reaction.

Correct AnswerverifiedThe first step is denaturation of the ta...View AnswerShow Answer

Restriction mapping is used to characterize cloned DNA.What does a restriction map tell the researcher about the cloned DNA?

Correct AnswerverifiedShow Answer

Fluorescence in situ hybridization is another way to visualize the presence of a nucleotide sequence.Which of the following is an advantage of FISH over Northern and Southern blots?

Correct AnswerverifiedShow Answer

ddATP,ddTTP,ddCTP,and ddGTP are labeled with red,green,yellow,and blue fluorescent dyes,respectively.A five- base read from a sequencing reaction produced the following color sequence,read by the computer: red,yellow,yellow,green,green.What is the sequence of the template DNA?

Correct AnswerverifiedShow Answer

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified
The gene of interest can be inserted int...
View Answer

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified
The first step is denaturation of the ta...
View Answer

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified