Question 1

This microscope uses two beams of light to produce a three- dimensional, color image.

Accepted Answer

A)   DIC microscope
B)   Darkfield microscope
C)   Fluorescence microscope
D)   Phase- contrast microscope
E)   None of the aboveF) D) and E)
G) C) and D)

Question 2

Assume you stain Bacillus by applying malachite green with heat and then counterstaining with safranin. Through the microscope, the green structures are

Accepted Answer

A)   Capsules.
B)   Flagella.
C)   Cell walls.
D)   Endospores.
E)   Can't tell.F) B) and E)
G) A) and C)

Question 3

Cells are differentiated after which step in the Gram stain?

Accepted Answer

A)   Alcohol- acetone
B)   Crystal violet
C)   Iodine
D)   SafraninE) B) and C)
F) A) and B)

Question 4

The purpose of a mordant in the Gram stain is

Accepted Answer

A)   To prevent the crystal violet from leaving the cells.
B)   To make the bacterial cells larger.
C)   To make the flagella visible.
D)   To remove the simple stain.
E)   None of the above.F) A) and E)
G) A) and C)

Question 5

The appearance of gram- negative bacteria after addition of the decolorizing agent in the Gram stain.

Accepted Answer

A)   Purple
B)   Red
C)   Colorless
D)   Brown
E)   None of the aboveF) B) and D)
G) A) and B)

Question 6

The purpose of the ocular lens is to

Accepted Answer

A)   Increase the light.
B)   Magnify the image from the objective lens.
C)   Improve resolution.
D)   Decrease the refractive index.
E)   None of the above.F) B) and C)
G) A) and E)

Question 7

Figure 3.1
 
-In Figure 3.1 line B points to the microscope's

Accepted Answer

A)   ocular lens.
B)   condenser.
C)   illuminator.
D)   objective lens.E) A) and B)
F) B) and D)

Question 8

Figure 3.1
 
-In Figure 3.1 line A points to the microscope's

Accepted Answer

A)   condenser.
B)   objective lens.
C)   ocular lens.
D)   illuminator.E) A) and B)
F) A) and C)

Question 9

Simple staining is often necessary to improve contrast in this microscope.

Accepted Answer

A)   Compound light microscope
B)   Phase- contrast microscope
C)   Darkfield microscope
D)   Fluorescence microscope
E)   Electron microscopeF) A) and C)
G) C) and D)

Question 10

Which of the following is not correct?

Accepted Answer

A)   1 µm = 10- 3 mm
B)   1 nm = 10- 6 µm
C)   1 µm = 10- 6 m
D)   1 µm = 103 nm
E)   1 nm = 10- 9 mF) A) and E)
G) B) and E)

Question 11

The signal molecule produced in quorum sensing is

Accepted Answer

A)   Simple stain.
B)   Counterstain.
C)   Inducer.
D)   Light.
E)   None of the above.F) A) and E)
G) B) and C)

Question 12

The appearance of gram- positive bacteria after addition of the first dye in the Gram stain.

Accepted Answer

A)   Purple
B)   Red
C)   Colorless
D)   Brown
E)   None of the aboveF) A) and B)
G) C) and D)

Question 13

In this microscope, the observer does not look at an image through a lens.

Accepted Answer

A)   Compound light microscope
B)   Phase- contrast microscope
C)   Darkfield microscope
D)   Fluorescence microscope
E)   Electron microscopeF) A) and E)
G) B) and E)

Question 14

The counterstain in the acid- fast stain is

Accepted Answer

A)   A mordant.
B)   A negative stain.
C)   A basic dye.
D)   An acid dye.
E)   Necessary to determine acid- fast cells.F) A) and C)
G) All of the above C

Question 15

This microscope is used to see internal structures of cells in a natural state.

Accepted Answer

A)   Compound light microscope
B)   Phase- contrast microscope
C)   Darkfield microscope
D)   Fluorescence microscope
E)   Electron microscopeF) A) and B)
G) A) and C) B

Question 16

The appearance of gram- negative bacteria after addition of the mordant in the Gram stain.

Accepted Answer

A)   Purple
B)   Red
C)   Colorless
D)   Brown
E)   None of the aboveF) A) and E)
G) All of the above A

Question 17

The counterstain in the Gram stain is

Accepted Answer

A)   A mordant.
B)   An acid dye.
C)   A negative stain.
D)   A basic dye.
E)   Necessary to determine the Gram reaction.F) B) and D)
G) A) and C)

Question 18

The appearance of gram- positive bacteria after adding the counterstain in the Gram stain.

Accepted Answer

A)   Purple
B)   Red
C)   Colorless
D)   Brown
E)   None of the aboveF) A) and D)
G) C) and D)

Question 19

The appearance of gram- negative bacteria after completing the Gram stain.

Accepted Answer

A)   Purple
B)   Red
C)   Colorless
D)   Brown
E)   None of the aboveF) B) and C)
G) A) and B)

Question 20

The best use of a negative stain is

Accepted Answer

A)   To determine cell size.
B)   To determine cell shape.
C)   To see endospores.
D)   To determine Gram reaction.
E)   A and BF) C) and D)
G) None of the above

Exam 3: Observing Microorganisms Through a Microscope

This microscope uses two beams of light to produce a three- dimensional, color image.

Correct AnswerverifiedShow Answer

Assume you stain Bacillus by applying malachite green with heat and then counterstaining with safranin. Through the microscope, the green structures are

Correct AnswerverifiedShow Answer

Cells are differentiated after which step in the Gram stain?

Correct AnswerverifiedShow Answer

The purpose of a mordant in the Gram stain is

Correct AnswerverifiedShow Answer

The appearance of gram- negative bacteria after addition of the decolorizing agent in the Gram stain.

Correct AnswerverifiedShow Answer

The purpose of the ocular lens is to

Correct AnswerverifiedShow Answer

Figure 3.1 -In Figure 3.1 line B points to the microscope's

Correct AnswerverifiedShow Answer

Figure 3.1 -In Figure 3.1 line A points to the microscope's

Correct AnswerverifiedShow Answer

Simple staining is often necessary to improve contrast in this microscope.

Correct AnswerverifiedShow Answer

Which of the following is not correct?

Correct AnswerverifiedShow Answer

The signal molecule produced in quorum sensing is

Correct AnswerverifiedShow Answer

The appearance of gram- positive bacteria after addition of the first dye in the Gram stain.

Correct AnswerverifiedShow Answer

In this microscope, the observer does not look at an image through a lens.

Correct AnswerverifiedShow Answer

The counterstain in the acid- fast stain is

Correct AnswerverifiedC

This microscope is used to see internal structures of cells in a natural state.

Correct AnswerverifiedB

The appearance of gram- negative bacteria after addition of the mordant in the Gram stain.

Correct AnswerverifiedA

The counterstain in the Gram stain is

Correct AnswerverifiedShow Answer

The appearance of gram- positive bacteria after adding the counterstain in the Gram stain.

Correct AnswerverifiedShow Answer

The appearance of gram- negative bacteria after completing the Gram stain.

Correct AnswerverifiedShow Answer

The best use of a negative stain is

Correct AnswerverifiedShow Answer

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified
C

Correct Answer
verified
B

Correct Answer
verified
A

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified