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The general transcriptional machinery is composed of the regulatory proteins bound to the promoter of a eukaryotic gene.

A) True
B) False

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The organization of the lac operon, from upstream to downstream sequences, is represented by:


A) repressor gene -> lacZ -> lacY -> lac A
B) lacZ -> lacY -> lac A
C) lacZ -> lacY -> lac A -> operator -> promoter
D) operator -> promoter -> lacZ -> lacY -> lac A
E) promoter -> operator -> lacZ -> lacY -> lac A

F) B) and E)
G) A) and D)

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Bacteria can respond quickly to their surroundings, such as in the presence of an abundant food source. This is because:


A) genes for functionally related enzymes are regulated together in operons.
B) genes for functionally related enzymes are always constitutive.
C) genes for functionally related enzymes are always under positive control.
D) efficient protein degradation removes unneeded proteins quickly.
E) all bacterial genes are constitutive.

F) A) and B)
G) D) and E)

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Long coding RNAs (lnc RNAs) are characterized by:


A) the presence of introns
B) the lack of a polyA tail
C) their roles in regulating transcription
D) being less than 100 bases in length
E) binding to zinc finger transcription factors

F) A) and B)
G) None of the above

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A repressible operon is usually controlled by:


A) an inactive repressor that allows it to be in the "on" state
B) the supply of the precursor product for the enzymes
C) an inactive repressor that keeps it in the "off" state
D) tryptophan
E) being turned "on," usually by the end product of the pathway

F) B) and C)
G) B) and E)

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Temporal gene regulation refers to which of the following circumstances?


A) Certain genes are induced only at a particular point in the organism's life cycle.
B) Certain genes are induced only in specific tissues.
C) Heat-shock genes are induced during periods of temperature stress.
D) "Housekeeping" genes are induced in response to a viral infection.
E) Molecular chaperones are synthesized after heavy-metal ingestion.

F) A) and B)
G) B) and E)

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Sketch the main elements of an inducible operon, such as the lactose operon, and explain the functions of the operator and promoter regions.

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Concepts to Consider: An operon is a set...

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Match the type of gene regulation with the process.

Premises
alternative splicing
Responses
regulation of transcription
regulation of translation
regulation of mRNA transport
regulation of mRNA processing
regulation of chromatin

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regulation of transcription
regulation of translation
regulation of mRNA transport
regulation of mRNA processing
regulation of chromatin

Many genes encode proteins that are always needed by a cell, and therefore are always expressed. Such genes are known as:


A) repressible
B) promoter
C) constitutive
D) inducible
E) operons

F) B) and C)
G) B) and D)

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Some eukaryotic DNA sequences act as introns in the cells of some tissues and exons in the cells of other tissues. This is an example of ____, and allows for ____.


A) positive control; feedback inhibition
B) gene amplification; the regulation of the rate of transcription
C) alternative splicing; the formation of different types of closely related proteins
D) DNA methylation; the formation of different types of closely related proteins
E) DNA replication; heterodimer formation

F) B) and D)
G) D) and E)

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How does the lactose repressor block transcription of the lactose operon?


A) by "turning off" the lacA intron
B) by degrading the lacZ protein product
C) by binding allosterically to the lacZ gene
D) by slowing the uptake of lactose into the cell
E) by binding to the operator

F) A) and C)
G) A) and D)

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A TATA box is seen in ____ cells and is the site where ____.


A) prokaryotic; RNA polymerase binds
B) eukaryotic; DNA ligase cleaves introns
C) eukaryotic; RNA polymerase binds
D) both prokaryotic and eukaryotic; transcription factors bind
E) prokaryotic; a repressor protein binds

F) C) and D)
G) B) and D)

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Enzymes that alter another protein or enzyme's activity by attaching a phosphate group are called:


A) kinases
B) phosphatases
C) phosphators
D) transcription factors
E) coenzymes

F) D) and E)
G) B) and D)

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An activator protein would have which of the following effects on an operon with a positive control mechanism?


A) stimulate transcription
B) turn off transcription
C) stimulate transcription when a coactivator is bound to the allosteric site
D) inhibit transcription when a coactivator is bound to the allosteric site
E) activator proteins only work on operons under negative control

F) None of the above
G) A) and B)

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Some transcription factors, such as leucine zipper proteins, form homodimers or heterodimers. How does this increase the complexity of available strategies of gene regulation?

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The example given in the text proposes t...

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Match the process with the corresponding description.

Premises
addition of phosphate groups by kinase enzymes
Responses
protein degradation
proteolytic processing
chemical modification

Correct Answer

protein degradation
proteolytic processing
chemical modification

Figure 14-1 Figure 14-1   The promoter in the accompanying figure is labeled as ____. A)  6 B)  3 C)  1 D)  2 E)  7 The promoter in the accompanying figure is labeled as ____.


A) 6
B) 3
C) 1
D) 2
E) 7

F) C) and D)
G) None of the above

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Explain genomic imprinting and how the phenotype can vary based on paternal or maternal inheritance of a particular allele.

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Concepts to consider: While most genomic...

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Constitutive genes code for proteins that are needed for survival.

A) True
B) False

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Figure 14-1 Figure 14-1   Refer to the accompanying figure. The structure labeled as 7 in the figure of the tryptophan operon is a(n) : A)  active repressor protein B)  inactive repressor protein C)  active RNA polymerase D)  inactive RNA polymerase E)  ribosome Refer to the accompanying figure. The structure labeled as 7 in the figure of the tryptophan operon is a(n) :


A) active repressor protein
B) inactive repressor protein
C) active RNA polymerase
D) inactive RNA polymerase
E) ribosome

F) B) and E)
G) A) and B)

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