Filters
Question type
Study Flashcards

A histotechnician receives a sample of bone marrow for biopsy. What fixative of choice is suitable for bone marrow preservation?


A) Alcohol
B) B-5 fixative
C) Carnoy's fluid
D) Orth's fluid

E) C) and D)
F) A) and C)

Correct Answer

verifed

verified

What are the characteristics of a good fixative?


A) It must inhibit bacterial decomposition and autolysis.
B) It must permit rapid and even penetration of tissues.
C) Both A and B
D) None of the above

E) B) and D)
F) A) and D)

Correct Answer

verifed

verified

One disadvantage of using perfusion as a fixation process is:


A) It is a costly process
B) It makes the subject paralyzed
C) The volume of fixative used is smaller
D) The tissue does not die until it is fixed

E) A) and D)
F) A) and B)

Correct Answer

verifed

verified

All of the following choices below are fixatives of choice for electron microscopy, except:


A) 100% Methanol
B) Osmium Tetroxide
C) Glutaraldehyde
D) Paraformaldehyde

E) A) and D)
F) All of the above

Correct Answer

verifed

verified

Among the choices below, which of the following fixatives is preferred for the preservation of mucopolysaccharides and nuclear proteins?


A) Carnoy's Fluid
B) 100% Methyl Alcohol
C) Newcomer's Fluid
D) Bouin's Solution

E) None of the above
F) A) and B)

Correct Answer

verifed

verified

What is the usual fixation time when using 10% formaldehyde?


A) 3 hours
B) 12 hours
C) 24 hours
D) 72 hours

E) A) and B)
F) A) and C)

Correct Answer

verifed

verified

It is the fixative of choice used to preserve touch preparations and for staining procedures of Wright- Giemsa.


A) Acetone
B) Formalin
C) Isopropyl alcohol
D) Osmic acid

E) A) and C)
F) A) and B)

Correct Answer

verifed

verified

It is an excellent fixative that is chiefly recommended for tumor biopsies especially of the skin.


A) Heidenhain's Susa Solution
B) Helly solution
C) B-5 Fixative
D) Zenker's Fluid

E) A) and B)
F) None of the above

Correct Answer

verifed

verified

Which among the following statements is not true about penetration?


A) The rate of penetration is always affected by heat.
B) The penetration of fixatives can be improved by opening up a tissue sample.
C) The concentration of the fixative always affects penetration.
D) The presence of intestinal contents can inhibit the penetration of fixatives.

E) None of the above
F) All of the above

Correct Answer

verifed

verified

What is this type of metallic fixative that produces black precipitates?


A) Mercuric chloride
B) Chromate fixative
C) Lead fixative
D) Heidenhain's Susa

E) B) and D)
F) B) and C)

Correct Answer

verifed

verified

As a histotechnician, one must know the basics of fixation before it occurs. One of the basic principles to remember is the ideal size of the tissue. Therefore, what is the ideal size of the tissue before fixing it?


A) 0) 5 cm2
B) 1 cm2
C) 2 cm2
D) 3 cm2

E) A) and B)
F) A) and C)

Correct Answer

verifed

verified

This type of picric acid fixative is recommended for the preservation of embryos and pituitary biopsies.


A) Bouin's solution
B) Brasil's Alcoholic picroformol
C) Glacial acetic acid
D) Carnoy's fluid

E) A) and D)
F) A) and C)

Correct Answer

verifed

verified

What is the suitable pH for fixation?


A) pH 2 to 3
B) pH 4 to 6
C) pH 6 to 8
D) pH 7 to 9

E) B) and C)
F) C) and D)

Correct Answer

verifed

verified

Heat fixation should be used on the following except for:


A) Gram stain
B) Capsular staining
C) Endospore staining
D) Acid Fast stain

E) All of the above
F) A) and C)

Correct Answer

verifed

verified

What substance is added to formaldehyde in order to remove the precipitate produced by prolonged storage?


A) Acetone
B) Acetic acid
C) Methanol
D) Distilled water

E) A) and B)
F) A) and C)

Correct Answer

verifed

verified

Which of the following is not an example of a nuclear fixative?


A) Flemming's fluid
B) Newcomer's fluid
C) Heidenhain's Susa
D) Orth's fluid

E) None of the above
F) A) and C)

Correct Answer

verifed

verified

This fixative is preferred for the preservation of chromosomes, lymph glands and urgent biopsies, as well as to preserve brain tissue for the identification of rabies.


A) Carnoy's Fluid
B) 100% Methyl alcohol
C) Bouin's Solution
D) Muller's Fluid

E) B) and C)
F) B) and D)

Correct Answer

verifed

verified

Which of the following is used as dehydrating agent and as a fixative at the same time?


A) Acetone
B) Mercuric Chloride
C) Acetic acid
D) Both A and B

E) B) and C)
F) A) and D)

Correct Answer

verifed

verified

Which of the following statements does not accurately describe formaldehyde?


A) It penetrates the tissues rapidly
B) Formaldehyde fixes tissue specimens at a rapid rate of about 1mm per hour.
C) It is an ideal fixative for nucleic acids
D) It is one of the most commonly used aldehyde fixative

E) A) and B)
F) None of the above

Correct Answer

verifed

verified

Which among the following is a physical method of fixation?


A) Microwave fixation
B) Cryo-preservation
C) Immersion fixation
D) Both A and B

E) A) and B)
F) A) and C)

Correct Answer

verifed

verified

Showing 21 - 40 of 63

Related Exams

Exam 2

Laboratory Operations

45 Questions

Exam 3

Microtomy

30 Questions

Exam 4

Processing and Embedding

30 Questions

Exam 5

Staining

136 Questions

Show Answer